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Michael Love
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DESeq2 time course with two genotypes
deseq2
timecourse
michael love
multiple time points
5.1 years ago
cp1015
• 0
0
votes
4
replies
854
views
DESeq2 Design Formula
DESeq2
michael love
updated 5.9 years ago by
Michael Love
42k • written 5.9 years ago by
gkuffel
▴ 10
0
votes
1
reply
1.3k
views
DESeq2 PCA plotting
deseq2
michael love
updated 6.0 years ago by
Michael Love
42k • written 6.0 years ago by
sam.far.7
• 0
1
vote
11
replies
1.9k
views
Need help with DESeq2's contrast feature
deseq2
michael love
updated 6.3 years ago by
Michael Love
42k • written 6.3 years ago by
Ashu
▴ 10
2
votes
3
replies
1.7k
views
DESeq2 for time series using time as continuous and looking at effect of time on DE
deseq2
timecourse
multiple time points
mlr
michael love
updated 6.4 years ago by
Michael Love
42k • written 6.4 years ago by
ap874
• 0
5
votes
10
replies
12k
views
Constructing tx2gene for Salmon txImport Quantification using Gencode Mouse Transcript Annotation
Michael Love
Salmon
tximport
tx2gene
updated 6.7 years ago by
Michael Love
42k • written 6.7 years ago by
pvd2107
▴ 30
6 results • Page
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Comment: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
If you do it this way, the dispersion estimates would be much higher than they should (as the cell type difference is not accounted for in …
Comment: ANOVA like approach of edgeR
by
SamGG
▴ 350
Simply remove the cell type from the model. design <- model.matrix(~targets$Status)
Answer: ANOVA like approach of edgeR
by
Yunshun Chen
▴ 870
You could try the followings: > design <- model.matrix(~ 0 + group) > contrast <- makeContrasts(PvsL = 0.5*(L.pregnant + B.pregnan…
Answer: Identical samples after deseq2 batch effect removal
by
maripane
• 0
Thanks Michael, so is there a way to work around this issue? Can I somehow make sure I can remove this batch effects without overcorrecti…
Comment: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
by
rcreed
• 0
The updated version worked well on my data. Thank you!
Votes
A: ANOVA-like test with edgeR
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
Answer: CleanUpRNAseq with a GTF that is missing mitochondria annotation data
A: TopGO: How to retrieve the gene list related to a GO ID ?
A: Error in DESeqDataSet : some values in assay are not integers
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